To provide a portion of the eating plan categories of the fresh new spoonbill, we made use of the isotopic thinking out-of possible target together with fish, crustaceans, and you will aquatic insect larvae accumulated out-of freshwater and intertidal habitats for the South Korea advertised when you look at the early in the day knowledge (Fig 1A, Dining table 2)

Feather examples was indeed eliminated to remove oils otherwise mud which could change the value of the brand new secure isotope proportion that with one another a beneficial chloroform:methanol 2:1 solution and you will soap, due to the fact an earlier analysis revealed that cleanup feathers having an excellent solvent may not be enough to make sure accuracy and you may repeatability out-of isotope investigation . Then, the newest tested feathers was basically eliminated which have detergent (Deconex sixteen also; Borer Chemie, Zuchwil, Switzerland), rinsed three times which have distilled water, and you will dehydrated in fume hood . The new wash examples was basically slash and you can homogenized that have stainless-steel scissors. ? 13 C and you can ? fifteen N analyses was indeed conducted using a continuous-flow stable isotope proportion spectrometer (IsoPrime-EA, Micromass, Manchester, UK) associated with a beneficial CN analyzer (NA Series dos, Ce Devices, Wigan, UK) at National Instrumentation Center to possess Environmental Management (NICEM) from the Seoul Federal College of your Republic off Korea. (more…)

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